DNA Probe for Lactobacillus delbrueckii.
نویسندگان
چکیده
From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognizes L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an alpha-P-labeled DNA probe.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 56 6 شماره
صفحات -
تاریخ انتشار 1990